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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has actually been noticed in many tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was found to affiliate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial function in promoting mobile-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are already explained. Two more splice variants had been reported but comprehensive nucleotide sequence information is just not nonetheless obtainable.

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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide generally is made up of the epitope regarded from the antibody.

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This manual outlines additional achievable optimization of cellular and immunoassay parameters to make certain the best possible success are...

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This manual outlines further more achievable optimization of cellular and immunoassay parameters to make certain the best possible benefits are...

Antibodies bound to the blocking peptide not bind for the epitope within the goal protein. By comparing the staining in the blocked antibody versus the antibody by itself, you can see which staining is specific.

Target Details Cyclin E belongs on the very conserved cyclin spouse and children, whose users are characterised by a spectacular periodicity in protein abundance with the mobile cycle. Cyclins function as regulators of CDK kinases. Diverse cyclins show distinct expression and degradation styles which add to the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates in the G1-S section boundary which is degraded as cells progress as a result of S stage.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...

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